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{"sheet":"sgRNA Oligo生成模板","index":0,"n_rows":103,"n_cols":7,"rows_kept":103,"cut":false,"hidden":false,"merged":[[0,0,0,3],[0,4,0,5]]}
["上海快易吉生物科技有限公司\n邮箱：sales@qegene.cn\n地址：上海松江区西林南路5号",null,null,null,"     使用说明： 此表格可以快速自动批量生成sgRNA快速克隆试剂盒所需的两条Oligo DNA片段，方便您发送至DNA合成公司合成片段，仅需填入基因名称和sgRNA序列即可自动生成Oligo DNA序列。使用此表格必须开启宏。"]
["序号","基因名称","sgRNA序列（20bp）","Oligo F：","Oligo F：5’-sgRNA序列gttttagagctagaaatagc-3’","Oligo R","Oligo R：5’-sgRNA反向互补序列cggtgtttcgtcctttccac-3’"]
["示例","ABC","AAAAAAAAAACCCCCCCCCC",{"$f":"B3&\"-F\"","v":"ABC-F"},{"$f":"C3&\"gttttagagctagaaatagc\"","v":"AAAAAAAAAACCCCCCCCCCgttttagagctagaaatagc"},{"$f":"B3&\"-R\"","v":"ABC-R"},{"$f":"seqrecom(C3)&\"cggtgtttcgtcctttccac\"","v":"GGGGGGGGGGTTTTTTTTTTcggtgtttcgtcctttccac"}]
[1,null,null,{"$f":"B4&\"-F\"","v":"-F"},{"$f":"C4&\"gttttagagctagaaatagc\"","v":"gttttagagctagaaatagc"},{"$f":"B4&\"-R\"","v":"-R"},{"$f":"seqrecom(C4)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[2,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C5)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[3,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C6)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[4,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C7)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[5,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C8)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[6,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C9)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[7,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C10)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[8,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C11)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[9,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C12)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[10,null,null,"-F",{"$f":"C13&\"gttttagagctagaaatagc\"","v":"gttttagagctagaaatagc"},"-R",{"$f":"seqrecom(C13)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[11,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C14)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[12,null,null,"-F",{"$f":"C15&\"gttttagagctagaaatagc\"","v":"gttttagagctagaaatagc"},"-R",{"$f":"seqrecom(C15)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[13,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C16)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[14,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C17)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[15,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C18)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[16,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C19)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[17,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C20)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[18,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C21)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[19,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C22)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[20,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C23)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[21,null,null,"-F",{"$f":"C24&\"gttttagagctagaaatagc\"","v":"gttttagagctagaaatagc"},"-R",{"$f":"seqrecom(C24)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[22,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C25)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[23,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C26)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[24,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C27)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[25,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C28)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[26,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C29)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[27,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C30)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[28,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C31)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[29,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C32)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[30,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C33)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[31,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C34)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[32,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C35)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[33,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C36)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[34,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C37)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[35,null,null,{"$f":"B38&\"-F\"","v":"-F"},"gttttagagctagaaatagc","-R",{"$f":"seqrecom(C38)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[36,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C39)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[37,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C40)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[38,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C41)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[39,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C42)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[40,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C43)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[41,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C44)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[42,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C45)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[43,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C46)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[44,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C47)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[45,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C48)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[46,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C49)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[47,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C50)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[48,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C51)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[49,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C52)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[50,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C53)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[51,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C54)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[52,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C55)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[53,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C56)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[54,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C57)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[55,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C58)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[56,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C59)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[57,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C60)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[58,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C61)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[59,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C62)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[60,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C63)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[61,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C64)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[62,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C65)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[63,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C66)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[64,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C67)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[65,null,null,{"$f":"B68&\"-F\"","v":"-F"},{"$f":"C68&\"gttttagagctagaaatagc\"","v":"gttttagagctagaaatagc"},{"$f":"B68&\"-R\"","v":"-R"},{"$f":"seqrecom(C68)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[66,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C69)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[67,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C70)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[68,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C71)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[69,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C72)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[70,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C73)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[71,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C74)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[72,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C75)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[73,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C76)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[74,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C77)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[75,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C78)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[76,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C79)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[77,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C80)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[78,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C81)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[79,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C82)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[80,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C83)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[81,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C84)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[82,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C85)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[83,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C86)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[84,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C87)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[85,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C88)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[86,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C89)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[87,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C90)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[88,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C91)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[89,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C92)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[90,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C93)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[91,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C94)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[92,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C95)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[93,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C96)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[94,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C97)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[95,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C98)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[96,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C99)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[97,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C100)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[98,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C101)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[99,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C102)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]
[100,null,null,"-F","gttttagagctagaaatagc","-R",{"$f":"seqrecom(C103)&\"cggtgtttcgtcctttccac\"","v":"cggtgtttcgtcctttccac"}]